Regulated Operon: narK-fnr

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
narK
- 3831350..3832537 nitrite extrusion protein COG2223 narK-BAC  
fnr
- 3830535..3831251 transcriptional regulator (FNR/CRP family) COG0664 x0456-BAC  

Operon evidence: Northern blotting (2.0 kb transcript)
Reference: Cruz Ramos H, et al. (1995)
Comments: Internal promoter in front of fnr, leading to a 0.7 kb monocistronic fnr transcript. A stem-loop structure between narK and fnr apparently does not function as a terminator


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
FNR Positive -52:-31 3832599..3832620 ACGTGTGATGTAATTCACAATC Cruz Ramos H, et al. (1995): DB PE
Reents H, et al. (2006): AR
SigA Promoter -52:+8 3832561..3832620 ACGTGTGATGTAATTCACAATCCTGTTTGGCTAGTTTTTGTATGATAAGACTGATTATTG Cruz Ramos H, et al. (1995): PE
ResD Positive -62:-44 3831324..3831341 CATTCACAAGATTGTTAG Nakano MM, et al. (1996): DB RG
Nakano MM, et al. (2001): RG
SigA Promoter -48:+18 3831262..3831327 TTAGTTTTTTCTCGATGGGTACAAATCAATTCACGTTACAATAAACACACAACGTGACTTTCTTCG Cruz Ramos H, et al. (1995): PE
NsrR Negative ND ND ND Nakano MM, et al. (2006): RG

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
AAAAACAAGGACAGCGGTGTCCTTGTTTTTTTACCTTGTG
     >>>>>>>>    <<<<<<<<
3830509..3830528
7..26
-12.8
fnr

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai