Regulated Operon: resABCDE

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
resA ypxA - 2419997..2420542 essential protein similar to cytochrome c biogenesis protein COG0526  
resB ypxB - 2418372..2420000
COG1333  
resC ypxC - 2417178..2418236
COG0755 resC-BAC  
resD ypxD - 2416375..2417097 two-component response regulator COG0745 resD-BAC-1 resD-BAC-2  
resE ypxE - 2414609..2416378 two-component sensor histidine kinase COG2770  

Operon evidence: Northern blotting
Reference: Sun G, et al. (1996), Sorokin A, et al. (1993), Azevedo V, et al. (1993)
Comments: Internal promoter in front of resD, leading to a 2.5 kb transcript. Azevedo found a 7.0 kb transcript that started upstream of ypuL (in front of resA), as well as a shorter 0.7 kb transcript corresponding to ypuL only. The transcripts detected by Azevedo and Sun both had a length of 7 kb, which is 1 kb longer than needed for a resABCDE operon. In addition, Azevedo found that a probe for ypuL hybridizes to the 7 kb transcript, indicating that the transcript corresponds to ypuL-resABCDE. Such a transcript agrees well with the length of 7 kb measured by Northern blotting. However, Sun's primer extension experiment showed a single promoter just in front of resA.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
PhoP Positive -72:-6 2420573..2420640 ACGCTTTCTAAATTTCACATAACCTTCAAAAAGTAAGAAATGTGAAATGAACGTGCAATGATATAATT Birkey SM, et al. (1998): GS FT
ResD Positive ND ND ND Sun G, et al. (1996): DB
Nakano MM, et al. (2001): RG
SigA Promoter -51:+4 2420563..2420617 CTTCAAAAAGTAAGAAATGTGAAATGAACGTGCAATGATATAATTGAATGGATCT Sun G, et al. (1996): PE
PhoP Negative -45:+132 2417097..2417166 CGACACAGTTCTCAAACTTTCTCACGATTTGATAAAATGAAAGTAACAGAAGGAAAGCAGGGGGAAAACA Birkey SM, et al. (1998): GS FT
SigA Promoter -43:+5 2417117..2417164 ACACAGTTCTCAAACTTTCTCACGATTTGATAAAATGAAAGTAACAGA Sun G, et al. (1996): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
AGAAACACCCGCTGACTGAGCGGGTGTTTTTTTAATAGCCA
     >>>>>>>>     <<<<<<<<
2414546..2414566
43..63
-16.9
resE

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai