Regulated Operon: spoIIGA-sigE-sigG

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
spoIIGA
+ 1603076..1604005 protease
 
sigE spoIIGB + 1604068..1604787 RNA polymerase sporulation-specific sigma-29 factor (sigma-E) COG1191 sigE-BAC  
sigG spoIIIG + 1604927..1605709 RNA polymerase sporulation-specific sigma factor (sigma-G) COG1191 sigG-BAC  

Operon evidence: promoter probe vectors; integrational plasmids; lacZ transcriptional fusions
Reference: Masuda ES, et al. (1988), Sun DX, et al. (1991), Kenney TJ & Moran CP Jr (1987)
Comments:

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -49:+19 1602982..1603049 TCAACATTAATTGACAGACTTTCCCACAGAGCTTGCTTTATACTTATGAAGCAAGAAGGGGAACAGCG Kenney TJ, et al. (1989): PE
Spo0A Positive -100:-86 1602931..1602945 ATACTTCCTCGACAA Baldus JM, et al. (1994): SDM RO FT
Fujita M, et al. (2005): RG OV GS
Spo0A Positive -85:-70 1602946..1602961 ATTAAGCAGATTTCCC Baldus JM, et al. (1994): SDM RO FT
Fujita M, et al. (2005): RG OV GS
Spo0A Positive -55:-38 1602976..1602993 TTCCTCTCAACATTAATT Baldus JM, et al. (1994): SDM RO FT
Fujita M, et al. (2005): RG OV GS
Spo0A Positive -41:-24 1602990..1603007 AATTGACAGACTTTCCCA Baldus JM, et al. (1994): SDM RO FT
Fujita M, et al. (2005): RG OV GS
RsfA Positive ND ND ND Juan Wu L, et al. (2000): RG DB
SigF Promoter -40:+13 1604857..1604909 GCAGTGCATATTTTTCCCACCCAAGGAGATACTTAACGTTGTACAGCAGCTCC Sun DX, et al. (1991): PE RG
SigG Promoter -40:+19 1604857..1604915 GCAGTGCATATTTTTCCCACCCAAGGAGATACTTAACGTTGTACAGCAGCTCCTGTAGG Sun DX, et al. (1991): PE RG
SpoVT Negative ND ND ND Bagyan I, et al. (1996): DB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
GAAAAGCCTTTAAAACGATGTTGTTTTAAAGGCTTTTCTATTGATTAT
     >>>>>>>>>>>>    <<<<<<<<<<<<
1605716..1605743
7..34
-16.3
 sigG

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai