Regulated Operon: spoIVCB-spoIIIC

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
spoIVCB cisB, sigK + 2652219..2652689 RNA polymerase sporulation-specific sigma factor (sigma-K) (N-terminal half) COG1191 sigK-BAC  
spoIIIC sigK, spoIVD, spoIVE + 2700564..2700980 RNA polymerase sporulation-specific sigma factor (sigma-K) (C-terminal half) COG1191 sigK-BAC sigK-BAC  

Operon evidence: upstream and downstream genes are in opposite directions
Reference: Stragier P, et al. (1989), Errington J, et al. (1988)
Comments: spoIVCB and spoIIIC form a composite gene in the mother cell during sporulation by excising the intervening DNA sequence.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
GerE Negative -9:+22 2652171..2652201 ATACATTTACATATAGGCTTTTGCCTACATA Zheng L, et al. (1992): RO
Ichikawa H, et al. (1999): FT RG
GerE Negative -1:+31 2652179..2652210 ACATATAGGCTTTTGCCTACATACTTTTGTGG Zheng L, et al. (1992): RO
Ichikawa H, et al. (1999): FT RG
SigE Promoter -39:+9 2652141..2652188 CGGTACAGACACAGACAGCCTCCCGGTCACATACATTTACATATAGGC Kunkel B, et al. (1988): PE RG
SigK Promoter -39:+9 2652141..2652188 CGGTACAGACACAGACAGCCTCCCGGTCACATACATTTACATATAGGC Kunkel B, et al. (1988): PE RG
Kroos L, et al. (1989): RO, SDS-PAGE
SpoIIID Positive -36:-16 2652144..2652164 TACAGACACAGACAGCCTCCC Halberg R, et al. (1994): FT RO
SpoIIID Positive +78:+100 2652257..2652279 TAAAGAGCTTGTCTTTTTAGTAT Halberg R, et al. (1994): FT RO
SpoIIID Positive +109:+133 2652288..2652312 AAAAACAATGCCTTTCCACAACCGC Halberg R, et al. (1994): FT RO

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAGAAGCCGGATCTCATACTCCGGCTTTCTCTATTTGAAA
     >>>>>>        <<<<<<
2700991..2701010
11..30
-11.6
 spoIIIC

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai