Regulated Operon: trePAR

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
treP treB + 849703..851115 phosphotransferase system (PTS) trehalose-specific enzyme IIBC component COG1264 treB-BAC  
treA treC + 851186..852871 trehalose-6-phosphate hydrolase COG0366 treC-BAC  
treR yfxA + 852892..853608 transcriptional regulator (GntR family) COG2188 treR-BAC  

Operon evidence: Northern blotting
Reference: Schock F & Dahl MK (1996), Schock F & Dahl MK (1996), BSORF
Comments: Schock measured a trePA (3.2 kb) and a trePAR (more than 4 kb; weak) transcript. The short transcript was hybridized to a treA probe only; it was identified as a trePA transcript based on its length. Northern blotting results in BSORF show a trePAR, a treAR, and a treR transcript. The terminator proposed by Schock lacks a T-stretch.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
CcpA Negative +362:+384 850018..850040 GCTGTGAAAACGCTTGCAGATAT Miwa Y, et al. (2000): RG
SigA Promoter -38:+4 849619..849660 GTGTTGACTACCTGTATATACAGGAATACAATATGATTATAA Schock F & Dahl MK (1996): PE
TreR Negative -38:-5 849619..849652 GTGTTGACTACCTGTATATACAGGAATACAATAT Schock F & Dahl MK (1996): DB GS HB
TreR Negative +6:+17 849652..849683 TGATTATAAGTTGTATATACAAGTTATAAAAA Schock F & Dahl MK (1996): DB GS HB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
GAGAGGCGCCTGCCTGCGGCAGCGCGCTTTTTTGTTTGGTAT
     >>>>>>>>>   <<<<<<<<<<
853614..853635
6..27
-16.8
treR

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai