Regulated Operon: ybaK-cwlD

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
ybaK ybxH + 156108..156551

 
cwlD
+ 156611..157324 N-acetylmuramoyl-L-alanine amidase COG0860 cwlD-BAC  

Operon evidence: Northern blotting (1.2 kb transcript)
Reference: Sekiguchi J, et al. (1995)
Comments: Internal promoter in front of cwlD, leading to a 0.73 kb transcript.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
LexA Negative -124:-101 155964..155987 ATTACAGAACATTTGTTCCTCACC Au N, et al. (2005): GS AR DB
SigG Promoter -42:+5 156046..156092 AATTCGGGCATTGTTTCATCATCCAAACCTCAAAATAATCGGTAAAA Sekiguchi J, et al. (1995): PE NB DB
SigE Promoter -39:+10 156548..156596 GTAATCATATTTCCCGACCCTGTCCCATAGTTATGTAATAACGGACAAG Sekiguchi J, et al. (1995): PE NB DB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
GGAGACCCTCCGGAGTAATGGAGGGTTTTCTTGTGGTTCT
     >>>>>>>      <<<<<<<
157312..157331
-12..7
-13.3
cwlD

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai