Regulated Operon: dacF-spoIIAAB-sigF

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
dacF
- 2445094..2446263
COG1686M dacF-BAC-1 dacF-BAC-2  
spoIIAA
- 2444645..2444998
COG1366T spoIIAA-BAC  
spoIIAB
- 2444208..2444648
COG2172T x1348-CLO  
sigF spoIIAC - 2443429..2444196
COG1191K sigF-BAC  

Operon evidence: Northern blotting (2.9 kb transcript)
Reference: Wu JJ, et al. (1989), Wu JJ, et al. (1992), BSORF
Comments: Internal promoter in front of spoIIAA, leading to a 1.7 kb transcript.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigF Promoter -37:+3 2446286..2446325 GGCGTATAAAACCATCACGCTTGGAAAAAATAAAAAGGAT Wu JJ, et al. (1992): NB PE
Schuch R & Piggot PJ (1994): RG
SigG Promoter -37:+3 2446286..2446325 GGCGTATAAAACCATCACGCTTGGAAAAAATAAAAAGGAT Schuch R & Piggot PJ (1994): DB RG OV
SpoVT Negative ND ND ND Bagyan I, et al. (1996): DB
SigH Promoter -44:+5 2445021..2445069 GTCACGGTGAAGGAATTCATTCCGTCGAAATCGAAACACTCATTATCCG Wu JJ, et al. (1989): PE DP RG DB
Wu JJ, et al. (1991): DP RG RO PE
Spo0A Positive -28:-13 2445038..2445053 TCATTCCGTCGAAATC Baldus JM, et al. (1994): FT
Fujita M, et al. (2005): OV RG GS
Spo0A Positive -51:-33 2445058..2445076 TAGTTTTGTCACGGTGAAG Baldus JM, et al. (1994): FT
Fujita M, et al. (2005): OV RG GS
Spo0A Positive -71:-52 2445077..2445096 TAATTATGCCGAATGACCAC Baldus JM, et al. (1994): FT
Fujita M, et al. (2005): OV RG GS
Spo0A Positive -66:-97 2445091..2445122 CGATGGGAGACTGGACAAAATTTAAGTAATTA Baldus JM, et al. (1994): FT
Fujita M, et al. (2005): OV RG GS

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
GGATGGCTAGTCTGCAGTGCAGGCTAGCTTTTTTGTGCAAAAG
     >>>>>>>>>>   <<<<<<<<<<
2443411..2443433
-4..18
-17.1
 sigF

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai