Regulated Operon: spoIIGA-sigE-sigG

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
spoIIGA
+ 1603779..1604708

 
sigE spoIIGB + 1604771..1605490
COG1191K sigE-BAC  
sigG spoIIIG + 1605630..1606412
COG1191K sigG-BAC  

Operon evidence: promoter probe vectors; integrational plasmids; lacZ transcriptional fusions
Reference: Masuda ES, et al. (1988), Sun DX, et al. (1991), Kenney TJ & Moran CP Jr (1987)
Comments:

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -49:+19 1603685..1603752 TCAACATTAATTGACAGACTTTCCCACAGAGCTTGCTTTATACTTATGAAGCAAGAAGGGGAACAGCG Kenney TJ, et al. (1989): PE
Spo0A Positive -100:-86 1603634..1603648 ATACTTCCTCGACAA Baldus JM, et al. (1994): SDM RO FT
Fujita M, et al. (2005): RG OV GS
Spo0A Positive -85:-70 1603649..1603664 ATTAAGCAGATTTCCC Baldus JM, et al. (1994): SDM RO FT
Fujita M, et al. (2005): RG OV GS
Spo0A Positive -55:-38 1603679..1603696 TTCCTCTCAACATTAATT Baldus JM, et al. (1994): SDM RO FT
Fujita M, et al. (2005): RG OV GS
Spo0A Positive -41:-24 1603693..1603710 AATTGACAGACTTTCCCA Baldus JM, et al. (1994): SDM RO FT
Fujita M, et al. (2005): RG OV GS
RsfA Positive ND ND ND Juan Wu L, et al. (2000): RG DB
SigF Promoter -40:+13 1605560..1605612 GCAGTGCATATTTTTCCCACCCAAGGAGATACTTAACGTTGTACAGCAGCTCC Sun DX, et al. (1991): PE RG
SigG Promoter -40:+19 1605560..1605618 GCAGTGCATATTTTTCCCACCCAAGGAGATACTTAACGTTGTACAGCAGCTCCTGTAGG Sun DX, et al. (1991): PE RG
SpoVT Negative ND ND ND Bagyan I, et al. (1996): DB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
GAAAAGCCTTTAAAACGATGTTGTTTTAAAGGCTTTTCTATTGATTAT
     >>>>>>>>>>>>    <<<<<<<<<<<<
1606419..1606446
7..34
-16.3
 sigG

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai