Regulated Operon: bmrU-bmr-bmrR

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
bmrU
+ 2492853..2493746 multidrug resistance protein COG1597  
bmr bmr1 + 2493847..2495016 multidrug-efflux transporter COG0477  
bmrR
+ 2495089..2495925 transcriptional regulator (MerR family)
 

Operon evidence: Northern blotting (3.8 kb transcript)
Reference: Petersohn A, et al. (1999), Ahmed M, et al. (1994), BSORF
Comments: Readthrough terminator downstream of bmrU leads to a 1.0 kb transcript. The readthrough terminator downstream of bmr leads to a 2.5 kb transcript. Internal promoter in front of bmr.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigB Promoter -40:+4 2492786..2492829 TCTTCGTTTACTGCTTACAGAAAAAGGGGATTATATAACCAGAA Petersohn A, et al. (1999): PE RG
BmrR Positive -37:-7 2493782..2493812 TTGACTCTCCCCTAGGAGGAGGTCTTACAGT Ahmed M, et al. (1994): DB GS FT
Mta Positive -35:-14 2493784..2493805 GACTCTCCCCTAGGAGGAGGTC Baranova NN, et al. (1999): NB FT
SigA Promoter -41:+4 2493778..2493822 TCCGTTGACTCTCCCCTAGGAGGAGGTCTTACAGTATAAGGGATA Baranova NN, et al. (1999): RO

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAAGAGCATTTTTTGAAACAAAACTTCAAAAAATGCTCTTTTTGCTTATTTAG
     >>>>>>>>>>>>>>       <<<<<<<<<<<<<<
2495941..2495975
16..50
-19.9
 bmrR
AAGAAGCGCATTCTTTGTGTACTGCAAAGAATGCGCTTCTTCTTTATACTG
     >>>>>>>>>>>>>     <<<<<<<<<<<<<
2495023..2495053
7..37
-23.3
 bmr

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai