Regulated Operon: hut

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
hutP hutP1 + 4040501..4040956 transcriptional regulator
hutP-BAC  
hutH
+ 4041069..4042595 histidase COG2986 hutH-BAC  
hutU
+ 4042592..4044250 urocanase COG2987 hutU-BAC  
hutI
+ 4044263..4045528 imidazolone-5-propionate hydrolase COG1228 hutI-BAC x1372-BAC  
hutG
+ 4045521..4046480 formiminoglutamate hydrolase COG0010 x0909-STA  
hutM
+ 4046556..4047983 histidine permease COG0833  

Operon evidence: Northern blotting (8.0 kb transcript); 5' primer extension analysis of hutP, hutH, and hutU
Reference: Yoshida K, et al. (1995), Yoshida K, et al. (2000), Oda M, et al. (2004), Oda M, et al. (1988), Wray LV Jr & Fisher SH (1994), Oda M, et al. (2000)
Comments: The stem-loop downstream of hutP is part of a terminator/antiterminator structure mediated by HutP, leading to a 0.6 kb transcript.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
AbrB Positive +199:+221 4040667..4040689 CAATTGAAACCGCTTCCAAAAAG Fisher SH, et al. (1994): FT
CcpA Negative +2:+15 4040470..4040483 GTTAATAGTTATCA Wray LV Jr, et al. (1994): SDM DP
CcpA Negative +203:+216 4040671..4040684 TGAAACCGCTTCCA Wray LV Jr, et al. (1994): SDM DP
CodY Negative -3:+23 4040466..4040491 CTCAGTTAATAGTTATCAGAATTTTT Fisher SH, et al. (1996): DB DP
SigA Promoter -46:+17 4040423..4040485 AAAAAACCTTTTGACTTCTGCTGCTGAACCAATTAATATAATACTCAGTTAATAGTTATCAGA Wray LV Jr, et al. (1994): PE
HutP Positive Between hutP and hutH 4040977..4041028 CGATAGGGGGCTATGCGTGAAAACAGAAGTTCACAGCATAGCTCCTTTTTGT Wray LV Jr, et al. (1994): RO SDM
Oda M, et al. (2000): GS

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAAGCACACCCCGCTCAGCATGGGATGTGCTTTATTTTTTATTCT
     >>>>>>>>>         <<<<<<<<<
4047992..4048018
9..35
-13.7
 hutM
GATAGGGGGCTATGCGTGAAAACAGAAGTTCACAGCATAGCTCCTTTTTGTATGGGCGC
     >>>>>>>>>>>>>>>        <<<<<<<<<<<<<<<<
4040983..4041021
27..65
-25.3
 hutP

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai