Regulated Operon: sinIR

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
sinI
+ 2551678..2551851 antagonist of SinR
 
sinR sin, flaD + 2551885..2552220 transcriptional regulator COG1396 sinR-BAC  

Operon evidence: S1 mapping of the 3' end
Reference: Gaur NK, et al. (1988)
Comments: Termination sites are the first and last T in the T-stretch TCTTTTGT of the first terminator, and the first T in the T-stretch TCTCTTTTTTT of the second terminator. Internal promoter in front of sinR.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
AbrB Negative +24:+46 2551579..2551601 TTTAATGGCAAATGACTTCCAGA Strauch MA (1995): GS
Shafikhani SH, et al. (2002): PE FT
Shafikhani SH & Leighton T (2004): DB RG
Hpr Negative -86:-57 2551470..2551499 ATCTGCAAAATAATATTTCAAGCTAACTTT Kallio PT, et al. (1991): FT
Shafikhani SH, et al. (2002): FT RG
Hpr Negative -10:+14 2551544..2551569 TATAATAAAGGTATATTGGAAAAAAA Kallio PT, et al. (1991): FT
Shafikhani SH, et al. (2002): FT RG
SigA Promoter -53:+7 2551503..2551562 ACCATTCGACATCATTCTCGTTTTTTTTGAGAAAATACGATTATAATAAAGGTATATTGG Gaur NK, et al. (1988): PE HM
Shafikhani SH, et al. (2002): PE
SigA Promoter -41:+5 2551584..2551629 TGGCAAATGACTTCCAGAGACTAATGAAGCATACAATAAGTCATGG Gaur NK, et al. (1988): PE HM
Spo0A Positive -50:-26 2551503..2551530 ACCATTCGACATCATTCTCGTTTTTTTT Shafikhani SH, et al. (2002): PE FT
SigA Promoter -49:+11 2551821..2551880 AGCCAGAAGTCATACCGTAAATCCTTTCTGAATGTGCTATAATATCACAAGGAAGGTGAT Gaur NK, et al. (1988): PE HM
Shafikhani SH, et al. (2002): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAAGAGGAGTAGTGCCTGAGCAGAGGCACTAACTCCTCTTTTGTCAATAACCA
     >>>>>>>>>>>>>>      <<<<<<<<<<<<<<<
2552212..2552246
-8..26
-25.3
 sinR
CCATCGAGACGGCCCAGTATATGAATACTGGGCCGTCTCTTTTTTTTGCTGTTA
     >>>>>>>>>>>>>>>    <<<<<<<<<<<<<<<
2552264..2552297
44..77
-31.2
 sinR

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai