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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | srfAA-srfAB-comS-srfAC-srfAD |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| srfAA | comL | + | 376525..387291 | surfactin synthetase | COG1020 | |
| srfAB | comL | + | 387304..398067 | surfactin synthetase | COG1020 | |
| comS | + | 390440..390580 | ||||
| srfAC | comL | + | 398104..401928 | surfactin synthetase | COG1020 | |
| srfAD | comL | + | 401957..402685 | surfactin synthetase | COG3208 |
| Operon evidence: | lacZ insertions |
|---|---|
| Reference: | Cosmina P, et al. (1993), Nakano MM, et al. (1991) |
| Comments: | The lacZ insertions show that the length of the operon is at least 25 kb, which covers srfAA-srfAB-comS-srfAC. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| CodY | Negative | -38:+49 | 376197..376283 | CGGTGATAAAAACATTTTTTTCATTTAAACTGAACGGTAGAAAGATAAAAAATATTGAAAACAATGAATAAATAGCCAAAATTGGTT |
Wray LV Jr, et al. (1996): DB FT GS |
| ComA | Positive | -118:-81 | 376117..376154 | TTGCGGCATCCCGCAAAAAATATTGCTGTAAATAAACT |
Hahn J & Dubnau D (1991): DB RG Nakano MM, et al. (1991): DP SDM Roggiani M & Dubnau D (1993): GS FT |
| ComA | Positive | -74:-37 | 376161..376198 | TTTCGGCATCCCGCATGAAACTTTTCACCCATTTTTCG |
Hahn J & Dubnau D (1991): DB RG Nakano MM, et al. (1991): DP SDM Roggiani M & Dubnau D (1993): GS FT |
| PerR | Positive | -163:-113 | 376072..376122 | TTTAAAAATTTTTATTTTTCTGTAAATAATGTTTAGTGGAAATGATTGCGG |
Hayashi K, et al. (2005): GS FT |
| SigA | Promoter | -46:+17 | 376189..376251 | CCATTTTTCGGTGATAAAAACATTTTTTTCATTTAAACTGAACGGTAGAAAGATAAAAAATAT |
Nakano MM, et al. (1991): PE |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| CAAAAGCGGACAGCTTCGGCTGTTCCGCTTTTTTTGTGTTGAA >>>>>>>>> <<<<<<<<<< |
srfAD |
|