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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | aprE |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| aprE | sprE | - | 1103727..1104872 | serine alkaline protease (subtilisin E) | COG1404 |
| Operon evidence: | Northern blotting; upstream and downstream genes are on the opposite strand |
|---|---|
| Reference: | Genbank Y14083, Ferrari E, et al.: Genetics and biotechnology of Bacilli, vol.2 pp.39-44 (1988) |
| Comments: |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| AbrB | Negative | -59:+15 | 1104917..1104989 | AAAATCATCTCAAAAAAATGGGTCTACTAAAATATTATTCCATCTATTACAATAAATTCACAGAATAGTCTTT |
Ferrari E, et al. (1986): DB RG Ferrari E, et al. (1988): Genetics and biotechnology of Bacilli, vol.2 pp.39-44: DP NB Strauch MA, et al. (1989): GS FT Ogura M, et al. (2003): DB RG |
| DegU | Positive | -83:-24 | 1104949..1105008 | GATATACCTAAATAGAGATAAAATCATCTCAAAAAAATGGGTCTACTAAAATATTATTCC |
Kunst F, et al. (1974): serine protease activity; DB Ayusawa D, et al. (1975): protease activity; DB Yoneda Y & Maruo B (1975): serine protease activity; DB Ferrari E, et al. (1986): DB RG Tanaka T & Kawata M (1988): serine protease activity; DB Henner DJ, et al. (1988): Genetics and biotechnology of Bacilli, vol.2 pp.3-9: DP RG Mukai K, et al. (1992): DP DB OV RG Ogura M, et al. (2003): RG DB GS Shimane K & Ogura M (2004): SDM DB RG |
| Hpr | Negative | -324:-295 | 1105225..1105254 | AGTTTATTTTTCAGAATACTTTTATCATCA |
Higerd TB, et al. (1972): serine protease activity Ferrari E, et al. (1986): DB RG Henner DJ, et al. (1988): DP RG DB NB PE Henner DJ, et al. (1988): Genetics and biotechnology of Bacilli, vol.2 pp.3-9: DP RG Kallio PT, et al. (1991): GS FT |
| Hpr | Negative | -292:-267 | 1105197..1105222 | CTTTGAAAAAATATCACGATAATATC |
Higerd TB, et al. (1972): serine protease activity Ferrari E, et al. (1986): DB RG Henner DJ, et al. (1988): DP RG DB NB PE Henner DJ, et al. (1988): Genetics and biotechnology of Bacilli, vol.2 pp.3-9: DP RG Kallio PT, et al. (1991): GS FT |
| Hpr | Negative | -79:-59 | 1104989..1105009 | TGATATACCTAAATAGAGATA |
Higerd TB, et al. (1972): serine protease activity Ferrari E, et al. (1986): DB RG Henner DJ, et al. (1988): DP RG DB NB PE Henner DJ, et al. (1988): Genetics and biotechnology of Bacilli, vol.2 pp.3-9: DP RG Kallio PT, et al. (1991): GS FT |
| Hpr | Negative | -35:-14 | 1104944..1104965 | TACTAAAATATTATTCCATCTA |
Higerd TB, et al. (1972): serine protease activity Ferrari E, et al. (1986): DB RG Henner DJ, et al. (1988): DP RG DB NB PE Henner DJ, et al. (1988): Genetics and biotechnology of Bacilli, vol.2 pp.3-9: DP RG Kallio PT, et al. (1991): GS FT |
| SigA | Promoter | -51:+18 | 1104913..1104981 | CTCAAAAAAATGGGTCTACTAAAATATTATTCCATCTATTACAATAAATTCACAGAATAGTCTTTTAAG |
Henner DJ, et al. (1988): PE NB DP Ferrari E, et al. (1988): RG PE DP NB Park SS, et al. (1989): DP S1, in-vitro transcription assay |
| SinR | Negative | -265:-220 | 1105150..1105195 | ATTGTTCTCACGGAAGCACACGCAGGTCATTTGAACGAATTTTTTC |
Gaur NK, et al. (1991): DP GS FT Olmos J, et al. (1997): DB RG Ogura M, et al. (2003): DB RG |
| TenA | Positive | ND | ND | ND |
Pang AS, et al. (1991): DB |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| AAAAAGAAGCAGGTTCCTCCATACCTGCTTCTTTTTATTTGTCAGC >>>>>>>>> <<<<<<<<< |
aprE |
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